Morphological differentiation between two parthenogentic populations of Artemia from Urmia Lake region
Behrooz Atashbar1,Alireza Asem2, Naser Agh1,Ramin Manaffar1
1. Artemia and Aquatic Animals Research Center, Urmia University, Urmia, IRAN
2. Department of Biology, Faculty of Science, Razi University, Kermanshah, IRANSummary:
Studying morphological characteristics is a useful too for differentiating different Artemia populations. Using this tool we tried to differentiate two parthenogentic populations of Artemia from Lake Urmia region. For this purpose cysts of two parthenogenetic populations of Artemia from Lake Urmia and Lagoons at the vicinity of the lake were hatched according to standard procedure (Sorgeloos, 1986). Newly hatched nauplii were transferred into cylindro-conical vessels containing brine water of 80 ppt and cultured till adulthood. Thirty adult females were randomly selected from each population and were killed using concentrated lugol solution. Thirteen morphologic characters were measured using a light microscope equipped with drawing tube and a digitizer. Moreover number of setae per furca were also counted. Two populations demonstrated significant differences (P<0.05) in six morphometric characters and also in numbers of seta per furca. Principal Components and Discriminante analysis were used to separate the populations On the basis of which these populations were separated with regard to factor 1 showing slight over lapping of the samples (Fig 1). On the basis of Discriminante analysis 98.3% of original grouped cases were correctly classified.Fig 1. Classification of populations by principal components analysis.
